OPTIMIZATION OF FEED-RATE IN FED-BATCH CULTURE ENTEROBACTER AEROGENES 17 E13 FOR MAXIMIZATION OF BIOMASS PRODUCTIVITY

Abstract

The paper presents the results of model-based optimization of fed-batch culture Enterobacter aerogenes 17 E13 for maximization of biomass productivity. A mathematical model for the problem solving is developed using mass-balance conditions for cells’ biomass and limiting substrate, relevant kinetic relationships for modeling the specific rates of biochemical transformations, and experimental data from fed-batch cultivation processes, realized under various cultivation conditions. The optimization procedure refers to the stochastic search algorithm based parametric optimization of flexible time function approximating the feed-rate time profile.